Journal: Frontiers in Oncology

Article Title: Development of an in vivo murine model of perineural invasion and spread of cutaneous squamous cell carcinoma of the head and neck

doi: 10.3389/fonc.2023.1231104

Figure Lengend Snippet: Live imaging and histological analysis of PNI of cutaneous SCCHN in NSG-A2 mice. (A) Live imaging of NSG-A2 mice on the first week post-primary tumor resection. Mice (n=8 (7 mice through experimental timeline, one imaged post-surgery but pre-euthanasia as noted (see Table 1 )) were injected with 15 mg/ml luciferin (IP), anesthetized with 2.5% isoflurane, and imaged. Lateral and superior images were taken at the highest magnification (Field of View: 4 cm). Images show the highest luminescence level representing luciferase saturation. Luminescence was measured using the IVIS imaging system, Living Image ® software v4.5.5. Radiance (p/sec/cm 2 /sr) represented by the colour scale is different for each mouse. Black arrows indicate a bioluminescence pattern suggestive of PNI that did not correlate with histology. Blue arrows indicated the bioluminescence patterns suggestive of secondary tumor growth on the neck region. (B) Region of Interest (ROI) measured by total flux (photons/second). Graphs were plotted using GraphPad Prism v8.3.1. After euthanasia, mouse heads were removed, fixed in formalin, and decalcified. Samples (n=7) were processed into paraffin wax. Three transverse sections (3-4 μm) were performed at 90°C at three different distances from the primary tumor site. Images show the second section displaying the nasal and oral cavity of animals. (C, G) H&E stain. (D, H) IHC stain for S100 (nerves). Sections were incubated with anti-S100 and secondary MACH1 Universal HRP anti-rabbit. (E, I) IHC stain for cytokeratin (cutaneous SCC). Incubation with anti-pan cytokeratin (AE1/AE3) was followed by secondary MACH2 HRP 221 anti-mouse. (F, J) Dual IHC for S100 and cytokeratin. S100 was stained with DAB (brown) and pan cytokeratin with Vector purple (purple). Arrowheads indicate intraneural and perineural invasion observed in mouse 8, and PNI in mouse 9. Data shown are the results of a single experiment. Slides were scanned at 40× magnification using the VS120 Olympus Slidescanner. Scale bars: 1000 µm and 100 µm (insets). Data were obtained from a single experiment. L, Lateral; S, Superior.

Article Snippet: This was followed by secondary incubation with MACH2 anti-mouse HRP (Biocare Medical) for 30 min at RT.

Techniques: Imaging, Injection, Luciferase, Software, Paraffin Wax, Staining, Incubation, Plasmid Preparation

Journal: Cell Metabolism

Article Title: Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19

doi: 10.1016/j.cmet.2020.11.005

Figure Lengend Snippet:

Article Snippet: MACH 2 Double Stain Kit 1 - Anti-Mouse AP/Anti-Rabbit HRP , BioCare Medical , Cat# MRCT523L.

Techniques: Plasmid Preparation, Control, Recombinant, Protease Inhibitor, Blocking Assay, Avidin-Biotin Assay, Staining, RNAscope, Multiplex Assay, Fluorescence, BIA-KA, Generated, Gene Expression, Software